Detection and removal of eyeblink artifacts from EEG using wavelet analysis and independent component analysis

Electrical signals generated by brain activity that are measured by the electroencephalogram can be distorted by electrical activity originating from eyeblinks and eye movements. This thesis proposes a new technique to identify and remove eyeblink artifacts from EEG data. An algorithm using a combination of wavelet analysis and independent component analysis (ICA) is implemented to detect the temporal location of the eyeblink artifact and eliminate it without compromising the integrity of the primary EEG data. The discrete wavelet transform is performed on 10 second epochs of data to detect the occurrence of ocular artifact. ICA is used to separate out the independent components within the data and the temporal locations of the eyeblink are used to remove the artifact and reconstruct the EEG data without that source of distortion. The results obtained indicate that the technique implemented may be robust enough to effectively process EEG data and is capable of removing eyeblink artifacts successfully when they are prominent and the data does not contain a great deal of movement artifact. The results show an 88.68% detection rate, a false positive rate of 4.03%, and an 87.23% removal rate for all eyeblinks that were accurately detected. The statistics obtained compared favorably with work done by others in this field of investigation

Zn/Ga−DFO iron–chelating complex attenuates the inflammatory process in a mouse model of asthma

Background: Redox-active iron, a catalyst in the production of hydroxyl radicals via the Fenton reaction, is one of the key participants in ROS-induced tissue injury and general inflammation. According to our recent findings, an excess of tissue iron is involved in several airway-related pathologies such as nasal polyposis and asthma. Objective: To examine the anti-inflammatory properties of a newly developed specific iron–chelating complex, Zn/Ga−DFO, in a mouse model of asthma. Materials and methods: Asthma was induced in BALBc mice by ovalbumin, using aluminum hydroxide as an adjuvant. Mice were divided into four groups: (i) control, (ii) asthmatic and sham-treated, (iii) asthmatic treated with Zn/Ga−DFO [intra-peritoneally (i/p) and intra-nasally (i/n)], and (iv) asthmatic treated with Zn/Ga−DFO, i/n only. Lung histology and cytology were examined. Biochemical analysis of pulmonary levels of ferritin and iron-saturated ferritin was conducted. Results: The amount of neutrophils and eosinophils in bronchoalveolar lavage fluid, goblet cell hyperplasia, mucus secretion, and peri-bronchial edema, showed markedly better values in both asthmatic-treated groups compared to the asthmatic non-treated group. The non-treated asthmatic group showed elevated ferritin levels, while in the two treated groups it returned to baseline levels. Interestingly, i/n-treatment demonstrated a more profound effect alone than in a combination with i/p injections. Conclusion: In this mouse model of allergic asthma, Zn/Ga−DFO attenuated allergic airway inflammation. The beneficial effects of treatment were in accord with iron overload abatement in asthmatic lungs by Zn/Ga−DFO. The findings in both cellular and tissue levels supported the existence of a significant anti-inflammatory effect of Zn/Ga−DFO